5YQ0
Crystal structure of secreted protein CofJ from ETEC.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 142.208, 147.540, 149.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.228 - 1.760 |
| R-factor | 0.1833 |
| Rwork | 0.180 |
| R-free | 0.21150 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.230 | 49.230 | 1.790 |
| High resolution limit [Å] | 1.760 | 9.640 | 1.760 |
| Rmerge | 0.126 | 0.048 | 2.016 |
| Rmeas | 0.135 | 0.052 | 2.164 |
| Rpim | 0.049 | 0.020 | 0.783 |
| Total number of observations | 2325718 | 14033 | 109819 |
| Number of reflections | 307221 | 2091 | 14749 |
| <I/σ(I)> | 10.6 | 28.6 | 1.3 |
| Completeness [%] | 99.5 | 99.4 | 97.6 |
| Redundancy | 7.6 | 6.7 | 7.4 |
| CC(1/2) | 0.998 | 0.996 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Tris-HCl pH 7.0, 14% PEG3350, 200mM CaCl2 |
