5YPJ

Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105N mutant).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	49.285, 48.536, 56.353
Unit cell angles	90.00, 95.00, 90.00

Refinement procedure

Resolution	28.630 - 2.010
R-factor	0.1617
Rwork	0.159
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yj3
RMSD bond length	0.018
RMSD bond angle	1.874
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.630	4.700	2.080
High resolution limit [Å]	2.010	4.330	2.010
Rmerge	0.045	0.022	0.210
Rmeas	0.061	0.030	0.283
Rpim	0.041	0.021	0.187
Number of reflections	17832	3458	3431
<I/σ(I)>	16.7
Completeness [%]	100.0	100	99.8
Redundancy	1.9	2	1.8
CC(1/2)	0.998	0.998	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 27% Peg3350, 3% Glycerol