5YPH
p62/SQSTM1 ZZ domain with Ile-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-02-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.658, 33.381, 35.019 |
Unit cell angles | 90.00, 103.61, 90.00 |
Refinement procedure
Resolution | 17.909 - 1.629 |
R-factor | 0.1511 |
Rwork | 0.147 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yp7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.305 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.910 |
High resolution limit [Å] | 1.629 |
Number of reflections | 9011 |
<I/σ(I)> | 34.7 |
Completeness [%] | 94.0 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | sodium phosphate, potassium phosphate, MgCl2 |