5YPF

p62/SQSTM1 ZZ domain with Trp-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	173
Detector technology	CCD
Collection date	2016-08-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.9
Spacegroup name	I 2 3
Unit cell lengths	114.964, 114.964, 114.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.646 - 2.951
R-factor	0.2438
Rwork	0.239
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.087
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.650
High resolution limit [Å]	2.953
Number of reflections	5464
<I/σ(I)>	50
Completeness [%]	99.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 2000, Tris