5YPE
p62/SQSTM1 ZZ domain with Tyr-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2016-02-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 3 |
Unit cell lengths | 114.541, 114.541, 114.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.496 - 2.851 |
R-factor | 0.2398 |
Rwork | 0.237 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.576 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.500 |
High resolution limit [Å] | 2.851 |
Number of reflections | 5991 |
<I/σ(I)> | 20.5 |
Completeness [%] | 100.0 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG 200, PEG 300, PEG 3350, Bis Tris Propane |