5YPE

p62/SQSTM1 ZZ domain with Tyr-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	173
Detector technology	CCD
Collection date	2016-02-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	I 2 3
Unit cell lengths	114.541, 114.541, 114.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.496 - 2.851
R-factor	0.2398
Rwork	0.237
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.500
High resolution limit [Å]	2.851
Number of reflections	5991
<I/σ(I)>	20.5
Completeness [%]	100.0
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 200, PEG 300, PEG 3350, Bis Tris Propane