5YPA

p62/SQSTM1 ZZ domain with Lys-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	173
Detector technology	CCD
Collection date	2015-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2
Spacegroup name	P 21 21 21
Unit cell lengths	29.085, 43.822, 67.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.993 - 2.502
R-factor	0.2167
Rwork	0.211
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.993	2.540
High resolution limit [Å]	2.502	2.500
Number of reflections	2939
<I/σ(I)>	13
Completeness [%]	96.1
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 8000, CaCl2, HEPES