5YP6
RORgamma (263-509) complexed with SRC2 and Compound 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-11-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.201, 62.201, 154.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.720 - 2.200 |
R-factor | 0.21464 |
Rwork | 0.213 |
R-free | 0.25446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l0j |
RMSD bond length | 0.005 |
RMSD bond angle | 0.820 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 57.720 |
High resolution limit [Å] | 2.200 |
Number of reflections | 15356 |
<I/σ(I)> | 24.19 |
Completeness [%] | 100.0 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Ammonium Sulfate, 0.1M Bis-Tris, pH 6.5, 14%(w/v) PEG 8000 |