5YOW

The post-fusion structure of the Heartland virus Gc glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 3
Unit cell lengths	117.875, 117.875, 117.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.292 - 2.100
R-factor	0.1853
Rwork	0.182
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g47
RMSD bond length	0.011
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rpim	0.032	0.495
Number of reflections	32160	3200
<I/σ(I)>	23.3	1.65
Completeness [%]	100.0	99
Redundancy	12.8	6.8
CC(1/2)		0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	6% (v/v) 2-propanol, 0.1M sodium acetate trihydrate, pH 4.5, 26%(v/v) polyethylene glycol monomethyl ether 550