5YOT
Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2016-05-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.141, 130.454, 94.836 |
| Unit cell angles | 90.00, 94.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.980 |
| R-factor | 0.16962 |
| Rwork | 0.167 |
| R-free | 0.21143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 50.000 | 2.032 |
| High resolution limit [Å] | 1.980 | 5.430 | 1.981 |
| Rmerge | 0.101 | 0.047 | 0.617 |
| Rmeas | 0.117 | 0.054 | 0.717 |
| Rpim | 0.057 | 0.026 | 0.361 |
| Number of reflections | 109351 | 5927 | 5388 |
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 93.9 | 99.9 | 93.2 |
| Redundancy | 3.9 | 4.2 | 3.7 |
| CC(1/2) | 0.994 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis-Tris pH6.5, 26% PEG MME550, 0.15M CaCl2 |
