5YOI

Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105T mutant) in complex with Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	49.236, 48.653, 56.052
Unit cell angles	90.00, 95.34, 90.00

Refinement procedure

Resolution	28.540 - 1.630
R-factor	0.16336
Rwork	0.161
R-free	0.21164
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yj3
RMSD bond length	0.021
RMSD bond angle	2.103
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.540	30.000	1.690
High resolution limit [Å]	1.630	3.510	1.630
Rmerge	0.034	0.026	0.232
Rmeas	0.046	0.036	0.308
Rpim	0.031	0.024	0.200
Number of reflections	33147		3323
<I/σ(I)>	26.5
Completeness [%]	97.8	99.9	94.3
Redundancy	1.9	1.9	1.8
CC(1/2)	0.998	0.997	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 27% Peg3350, 3% glycerol