5YOG

Crystal Structure of flavodoxin with engineered disulfide bond N14C-C93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-06
Detector	MAR CCD 130 mm
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	32.607, 56.321, 41.418
Unit cell angles	90.00, 101.63, 90.00

Refinement procedure

Resolution	32.917 - 1.420
R-factor	0.155
Rwork	0.153
R-free	0.17980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j8q
RMSD bond length	0.016
RMSD bond angle	1.558
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.917
High resolution limit [Å]	1.420
Number of reflections	27376
<I/σ(I)>	21.67
Completeness [%]	99.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	Tris-HCL buffer, pH 7.3 Ammonium Sulfate 3.1M