5YOE
Crystal Structure of flavodoxin with engineered disulfide bond A43C-L74C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-06 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.450, 56.132, 40.954 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 32.682 - 1.350 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j8q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.682 |
| High resolution limit [Å] | 1.350 |
| Number of reflections | 31057 |
| <I/σ(I)> | 26.21 |
| Completeness [%] | 98.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCL 0.1M Ammonium Sulfate 3.2M |
