5YOB
Crystal Structure of flavodoxin without engineered disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-06 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.232, 56.246, 41.058 |
Unit cell angles | 90.00, 100.84, 90.00 |
Refinement procedure
Resolution | 31.656 - 1.142 |
R-factor | 0.1668 |
Rwork | 0.166 |
R-free | 0.17740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j8q |
RMSD bond length | 0.009 |
RMSD bond angle | 1.088 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.656 |
High resolution limit [Å] | 1.142 |
Number of reflections | 38709 |
<I/σ(I)> | 32.21 |
Completeness [%] | 75.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCL 0.1M Ammonium Sulfate 3.2M |