5YNZ
Crystal structure of the dihydroorotase domain (K1556A) of human CAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2017-10-06 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 88.841, 108.630, 99.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.805 - 2.774 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c6b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.070 | 0.498 |
| Number of reflections | 12440 | 1201 |
| <I/σ(I)> | 23.68 | 2.74 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2M sodium chloride, 100mM MES sodium salt pH6.5, 200mM sodium acetate |
