5YNT

Crystal structure of an aromatic prenyltransferase FAMD1 from Fischerella ambigua UTEX 1903

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-08
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	93.398, 211.400, 82.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.790
R-factor	0.11192
Rwork	0.109
R-free	0.17062
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.850
High resolution limit [Å]	1.790	3.850	1.790
Rmerge	0.044	0.023	0.255
Rmeas	0.051	0.026	0.291
Rpim	0.024	0.013	0.139
Total number of observations	332166
Number of reflections	76749	7993	7528
<I/σ(I)>	10.7
Completeness [%]	99.9	99.8	99.3
Redundancy	4.3	4.2	4.3
CC(1/2)		0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	1.0 M sodium citrate, 0.1 M Imidazole pH 8.0