5YNL
Crystal structure of a cold-adapted arginase from psychrophilic yeast, Glaciozyma antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-07 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.193, 91.193, 107.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.767 - 2.349 |
| R-factor | 0.2264 |
| Rwork | 0.224 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hze |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.700 | 2.530 |
| High resolution limit [Å] | 2.349 | 2.350 |
| Number of reflections | 13850 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 95.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M sodium malonate pH 5, 12 % w/v polyethylene glycol 3,350, 0.1 M TCEP hydrochloride, 10mg/ml purified protein |
