5YNL

Structure of Glaciozyma antarctica Arginase Reveals Cold-active Adaptation via Increased Flexible Access to the Active Site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-07
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54178
Spacegroup name	H 3
Unit cell lengths	91.193, 91.193, 107.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.784 - 2.350
Rwork	0.221
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.285
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.700	2.530
High resolution limit [Å]	2.349	2.350
Number of reflections	13850	13850
<I/σ(I)>	1.6
Completeness [%]	95.0
Redundancy	3.7
CC(1/2)	0.772	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M sodium malonate pH 5, 12 % w/v polyethylene glycol 3,350, 0.1 M TCEP hydrochloride, 10mg/ml purified protein