5YMX

Myxococcus xanthus MglA in GDP bound conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	67.340, 68.610, 89.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.924 - 1.350
R-factor	0.152
Rwork	0.150
R-free	0.17870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t12
RMSD bond length	0.005
RMSD bond angle	0.878
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.030	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.055	0.734
Rmeas	0.060	0.791
Rpim	0.022	0.294
Number of reflections	91269	13188
<I/σ(I)>	14.6	2.6
Completeness [%]	99.7	100
Redundancy	7.2	7.2
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% 2-Methyl 2,4-pentane diol (MPD), 10% PEG 4000, 100mM Imidazole, pH 8.0