5YMT

Functional and structural characterization of P[19] rotavirus VP8* interaction with histo-blood group antigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	123
Detector technology	CCD
Collection date	2017-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 32
Unit cell lengths	131.667, 131.667, 150.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.907 - 2.199
R-factor	0.2281
Rwork	0.226
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gj6
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.199
Number of reflections	148275
<I/σ(I)>	15.231
Completeness [%]	99.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v polyethylene glycol MME 5000