5YMA
Crystal structure of ribosome assembly factor Efg1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-22 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 |
| Unit cell lengths | 99.122, 99.122, 90.179 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.755 - 3.295 |
| R-factor | 0.2035 |
| Rwork | 0.198 |
| R-free | 0.25170 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.295 | 3.300 |
| Rmerge | 0.362 | 1.000 |
| Number of reflections | 7701 | 385 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 22 | 20.1 |
| CC(1/2) | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.4M sodium malonate (pH 7.0) |
