5YM8
Crystal Structure of porcine delta coronavirus nsp9-N7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.546, 83.863, 36.344 |
Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
Resolution | 34.921 - 1.992 |
R-factor | 0.2161 |
Rwork | 0.214 |
R-free | 0.24760 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.990 |
Number of reflections | 605729 |
<I/σ(I)> | 24.51 |
Completeness [%] | 99.8 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1 M Na2HPO4:citric acid (pH 4.2), 22% (w/v) PEG 8000 |