5YM8
Crystal Structure of porcine delta coronavirus nsp9-N7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.546, 83.863, 36.344 |
| Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
| Resolution | 34.921 - 1.992 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.24760 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.990 |
| Number of reflections | 605729 |
| <I/σ(I)> | 24.51 |
| Completeness [%] | 99.8 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1 M Na2HPO4:citric acid (pH 4.2), 22% (w/v) PEG 8000 |
