5YM7
Crystal Structure of B562RIL without disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-17 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 41.847, 51.155, 89.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.975 - 1.562 |
R-factor | 0.201049806393 |
Rwork | 0.197 |
R-free | 0.23844 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6t |
RMSD bond length | 0.002 |
RMSD bond angle | 0.459 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.975 |
High resolution limit [Å] | 1.562 |
Number of reflections | 14000 |
<I/σ(I)> | 39.06 |
Completeness [%] | 100.0 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | NH4(SO4), Bicine , N-octanoylsucrose |