5YLY
Crystal structure of the cytochrome b5 reductase domain of Ulva prolifera nitrate reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.050, 96.220, 68.190 |
| Unit cell angles | 90.00, 96.72, 90.00 |
Refinement procedure
| Resolution | 17.740 - 1.760 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.218 | 1.764 |
| High resolution limit [Å] | 1.758 | 1.758 |
| Rmerge | 0.044 | 0.845 |
| Rmeas | 0.047 | 0.905 |
| Rpim | 0.017 | 0.323 |
| Number of reflections | 62093 | 593 |
| <I/σ(I)> | 25.8 | 2.2 |
| Completeness [%] | 97.4 | 96.4 |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 30% PEG 3350 (w/v), 200mM ammonium acetate, 100mM Bis-Tris pH 6.2 |
