5YLX

Integrated illustration of a valid epitope based on the SLA class I structure and tetramer technique could carry forward the development of molecular vaccine in swine species

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-08-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	66.058, 74.059, 98.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.607 - 2.200
R-factor	0.2023
Rwork	0.200
R-free	0.24320
RMSD bond length	0.014
RMSD bond angle	1.140
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.083	0.286
Number of reflections	24678
<I/σ(I)>	27.366	7.517
Completeness [%]	99.5	98.9
Redundancy	7.9	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277.15	0.1M Ammonium acetate, 0.1M BIS-TRIS pH5.5, 17% PEG 10000, temperature 277.15K