5YLL
Structure of GH113 beta-1,4-mannanase complex with M6.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-29 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.602, 69.960, 186.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.750 - 1.810 |
R-factor | 0.1868 |
Rwork | 0.186 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ylh |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (v1.12) |
Refinement software | PHENIX (v1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.750 | 1.880 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.086 | 0.086 |
Number of reflections | 68340 | |
<I/σ(I)> | 45.1 | 9.4 |
Completeness [%] | 91.0 | 91 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, HEPES Na, 2-Propylalcohol |