5YLA
Crystal structure of a dimeric peptidyl-tRNA hydrolase from Acinetobacter baumannii at 1.67 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.560, 98.100, 123.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.900 - 1.680 |
R-factor | 0.16229 |
Rwork | 0.161 |
R-free | 0.19437 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y98 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.898 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.900 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.061 | 0.793 |
Number of reflections | 49106 | |
<I/σ(I)> | 14.8 | 2.1 |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 4.8 | 4.9 |
CC(1/2) | 0.999 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50 mm Na-HEPES, PEG 1500, pH 7.5 |