5YL9

1.86 Angstrom crystal structure of human Coronavirus 229E fusion core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2017-03-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97945
Spacegroup name	H 3 2
Unit cell lengths	46.941, 46.941, 402.145
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.683 - 1.861
R-factor	0.1836
Rwork	0.182
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ieq
RMSD bond length	0.006
RMSD bond angle	0.632
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	1.930
High resolution limit [Å]	1.860	1.860
Rmerge	0.073	0.654
Rmeas	0.075	0.670
Rpim	0.016	0.143
Number of reflections	15101	1079
<I/σ(I)>	31.5	5.3
Completeness [%]	99.9	100
Redundancy	20
CC(1/2)	1.000	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	citric acid, BIS-TRIS propane, PEG3350