5YL1
T=1 subviral particle of Penaeus vannamei nodavirus capsid protein deletion mutant (delta 1-37 & 251-368)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-09 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 196.981, 200.319, 419.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.620 - 3.120 |
R-factor | 0.21959 |
Rwork | 0.220 |
R-free | 0.22071 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.696 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.620 | 3.230 |
High resolution limit [Å] | 3.120 | 3.120 |
Rmeas | 0.244 | 0.963 |
Rpim | 0.108 | 0.428 |
Number of reflections | 292229 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 5 | 5 |
CC(1/2) | 0.646 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Tris 8.0, 20%(w/v) Poly(acrylic acid sodium salt) 5100 |