5YL0
The crystal structure of Penaeus vannamei nodavirus P-domain (P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.843, 69.736, 146.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.260 - 1.220 |
| R-factor | 0.17695 |
| Rwork | 0.175 |
| R-free | 0.22039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.220 | |
| Rmeas | 0.128 | 0.490 |
| Rpim | 0.059 | 0.214 |
| Number of reflections | 134587 | |
| <I/σ(I)> | 38.6 | |
| Completeness [%] | 86.9 | 86.9 |
| Redundancy | 4.5 | 4.8 |
| CC(1/2) | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.075M Magnesium chloride hexahydrate, 0.1M Sodium cacodylate 6.5, 30%(w/v) PEG 2000 MME |
