5YKZ
The crystal structure of Penaeus vannamei nodavirus P-domain (P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.600, 53.192, 46.072 |
| Unit cell angles | 90.00, 109.38, 90.00 |
Refinement procedure
| Resolution | 22.500 - 1.170 |
| R-factor | 0.15159 |
| Rwork | 0.151 |
| R-free | 0.16984 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmeas | 0.088 | 0.467 |
| Rpim | 0.041 | 0.220 |
| Number of reflections | 65509 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.1 | 92.4 |
| Redundancy | 4.6 | 4.3 |
| CC(1/2) | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 30%(w/v) Polyethylene glycol 4,000, 100mM MES, pH 6.5 |
