5YKS

Crystal structure of sucrose nonfermenting-related kinase (SNRK)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	91.920, 99.075, 118.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.297 - 2.900
R-factor	0.2241
Rwork	0.222
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fe3
RMSD bond length	0.008
RMSD bond angle	0.977
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.497
Rmeas	0.087	0.534
Rpim	0.033	0.194
Number of reflections	23940
<I/σ(I)>	40.2	5.15
Completeness [%]	96.2	99.9
Redundancy	6.8	7.4
CC(1/2)		0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1 M HEPES, 0.07 M Magnesium formate, 4.5% D-Sorbitol