5YKP
Human methionine aminopeptidase type 1b (F309M mutant) in complex with ovalicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-07-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.713, 77.398, 47.489 |
Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
Resolution | 23.750 - 1.680 |
R-factor | 0.1768 |
Rwork | 0.175 |
R-free | 0.21170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gz5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.665 |
Data reduction software | DENZO (2.3.1) |
Data scaling software | SCALEPACK (2.2.0) |
Phasing software | MOLREP (11.5.05) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.000 | 27.000 | 1.740 |
High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
Rmerge | 0.070 | 0.044 | 0.501 |
Rmeas | 0.082 | 0.051 | 0.599 |
Rpim | 0.043 | 0.026 | 0.323 |
Number of reflections | 39533 | 4024 | 3892 |
<I/σ(I)> | 13.3 | ||
Completeness [%] | 99.9 | 99.8 | 99.2 |
Redundancy | 3.6 | 3.7 | 3.2 |
CC(1/2) | 0.997 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Hepes pH-6.0, 19% PEG MME 2000 |