5YKJ

Structural basis of the thiol resolving mechanism in yeast mitochondrial 1-Cys peroxiredoxin via glutathione/thioredoxin systems

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97022
Spacegroup name	P 41 21 2
Unit cell lengths	84.030, 84.030, 62.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.156 - 1.530
R-factor	0.1793
Rwork	0.178
R-free	0.19270
RMSD bond length	0.031
RMSD bond angle	1.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	1.580
High resolution limit [Å]	1.530	1.530
Number of reflections	34456	3385
<I/σ(I)>	14.24	3.87
Completeness [%]	100.0	99.91
Redundancy	22.5	18.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	23% (W/V) PEG-2000 MME ,100mM Tris base/Hydrochloric acid pH 4.7.