5YKC
crystal structure of H5 hemagglutinin from A/chicken/Taiwan/0502/2012
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.424, 220.103, 186.508 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.036 - 2.823 |
R-factor | 0.2011 |
Rwork | 0.197 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e2y |
RMSD bond length | 0.013 |
RMSD bond angle | 1.266 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.040 | 2.920 |
High resolution limit [Å] | 2.823 | 2.820 |
Rmerge | 0.074 | 0.685 |
Number of reflections | 46855 | |
<I/σ(I)> | 14.14 | |
Completeness [%] | 85.4 | 99.2 |
Redundancy | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M tris, 0.2M MgCl, 14% PEG8000 |