5YJZ
The native crystal structure of Rv3197 from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00849 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 187.290, 187.290, 67.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.390 - 2.160 |
R-factor | 0.1781 |
Rwork | 0.176 |
R-free | 0.20800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.062 | 0.270 |
Number of reflections | 37838 | 1817 |
<I/σ(I)> | 45.8 | 8.2 |
Completeness [%] | 99.8 | 97.7 |
Redundancy | 14.3 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.0M lithium sulfate, 0.1 M Tris (pH 8.5), 2% (w/v) PEG 400 |