5YJP
Human chymase in complex with 3-(ethoxyimino)-7-oxo-1,4-diazepane derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-05-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 43 |
Unit cell lengths | 75.089, 75.089, 49.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.610 - 1.800 |
R-factor | 0.15658 |
Rwork | 0.155 |
R-free | 0.18525 |
RMSD bond length | 0.033 |
RMSD bond angle | 2.486 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 24820 |
<I/σ(I)> | 14.3 |
Completeness [%] | 96.3 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 22% PEG8000, 5mM zinc chloride and 100mM MES buffer |