5YJP

Human chymase in complex with 3-(ethoxyimino)-7-oxo-1,4-diazepane derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-05-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 43
Unit cell lengths	75.089, 75.089, 49.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.610 - 1.800
R-factor	0.15658
Rwork	0.155
R-free	0.18525
RMSD bond length	0.033
RMSD bond angle	2.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.800
Number of reflections	24820
<I/σ(I)>	14.3
Completeness [%]	96.3
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	22% PEG8000, 5mM zinc chloride and 100mM MES buffer