5YJL
Crystal structure of Arabidopsis glutamyl-tRNA reductase in complex with NADPH and GBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.340, 83.757, 344.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.958 - 2.700 |
R-factor | 0.2165 |
Rwork | 0.213 |
R-free | 0.27370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n7r |
RMSD bond length | 0.009 |
RMSD bond angle | 1.116 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.958 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.126 | 0.687 |
Number of reflections | 48449 | |
<I/σ(I)> | 21.3 | |
Completeness [%] | 98.8 | 98.9 |
Redundancy | 9.2 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 22.5%(w/v) PEG 3,350, 0.3M ammonium citrate tribasic, pH 7.0, 0.4mM NADPH |