5YJL
Crystal structure of Arabidopsis glutamyl-tRNA reductase in complex with NADPH and GBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.340, 83.757, 344.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.958 - 2.700 |
| R-factor | 0.2165 |
| Rwork | 0.213 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.958 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.126 | 0.687 |
| Number of reflections | 48449 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 9.2 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 22.5%(w/v) PEG 3,350, 0.3M ammonium citrate tribasic, pH 7.0, 0.4mM NADPH |
