5YJ9
Crystal structure of Tribolium castaneum PINK1 kinase domain in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.028, 59.790, 50.822 |
| Unit cell angles | 90.00, 101.82, 90.00 |
Refinement procedure
| Resolution | 49.744 - 2.530 |
| R-factor | 0.2297 |
| Rwork | 0.229 |
| R-free | 0.24340 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.530 |
| Number of reflections | 13138 | |
| <I/σ(I)> | 21.7 | 1.95 |
| Completeness [%] | 93.7 | 94 |
| Redundancy | 9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | calcium acetate, PEG 400 |
