5YJ3
Crystal structure of TZAP and telomeric DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 56.713, 56.713, 226.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.279 - 2.845 |
R-factor | 0.2281 |
Rwork | 0.226 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w0t |
RMSD bond length | 0.004 |
RMSD bond angle | 0.590 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.920 |
High resolution limit [Å] | 2.845 | 7.020 | 2.850 |
Rmerge | 0.071 | 0.041 | 0.983 |
Rmeas | 0.073 | 0.042 | 1.007 |
Rpim | 0.015 | 0.009 | 0.214 |
Number of reflections | 9428 | 747 | 582 |
<I/σ(I)> | 4.8 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 23.7 | 19.3 | 21.2 |
CC(1/2) | 1.000 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Imidazole-Malate pH 8.5, 20% PEG 4K |