5YIV
Caulobacter crescentus GcrA DNA-binding domain(DBD) in complex with methylated dsDNA(crystal form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 124.017, 131.704, 77.664 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.030 - 2.914 |
R-factor | 0.2028 |
Rwork | 0.201 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yiu |
RMSD bond length | 0.004 |
RMSD bond angle | 0.728 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.110 | |
Rmeas | 0.120 | |
Rpim | 0.050 | |
Number of reflections | 13413 | 1010 |
<I/σ(I)> | 12.4 | 1.1 |
Completeness [%] | 94.3 | 92.2 |
Redundancy | 4.8 | |
CC(1/2) | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 30% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.2M Ammonium surface |