5YIM

Structure of a Legionella effector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	139.650, 295.430, 194.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.394
R-factor	0.2526
Rwork	0.251
R-free	0.28710
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.390	3.390
Number of reflections	55636
<I/σ(I)>	14.8
Completeness [%]	99.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Sodium formate