5YIK

Structure of a Legionella effector with its substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	108.846, 145.893, 104.088
Unit cell angles	90.00, 104.46, 90.00

Refinement procedure

Resolution	42.544 - 3.102
R-factor	0.2257
Rwork	0.223
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 5yim
RMSD bond length	0.004
RMSD bond angle	0.873
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	25234
<I/σ(I)>	16
Completeness [%]	99.7
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 1000, Tris