5YIJ

Structure of a Legionella effector with substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	107.779, 145.851, 103.349
Unit cell angles	90.00, 103.82, 90.00

Refinement procedure

Resolution	39.956 - 3.180
R-factor	0.2287
Rwork	0.226
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 5yim
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.180	3.180
Number of reflections	24125
<I/σ(I)>	25.9
Completeness [%]	99.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 1000, Tris