5YIF
Pyruvylated beta-D-galactosidase from Bacillus sp. HMA207, E163A mutant pyruvylated beta-D-galactose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.780, 114.780, 150.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.400 - 2.450 |
| R-factor | 0.20925 |
| Rwork | 0.206 |
| R-free | 0.27001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yhs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.081 | 0.855 |
| Number of reflections | 42682 | 2129 |
| <I/σ(I)> | 12.1 | 1.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.3 | 5.1 |
| CC(1/2) | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 32% PEG 300, 0.1 M phosphate-citrate |
