5YHY
Structure of Lactococcus lactis ZitR, C30S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.182, 62.182, 117.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.216 - 1.650 |
| R-factor | 0.2067 |
| Rwork | 0.206 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5GYS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.844 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.077 | 0.973 |
| Number of reflections | 36287 | 3542 |
| <I/σ(I)> | 29.2 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.19mM CYMAL-7, 0.1M HEPES pH 7.5, 40%(v/v) Polyethylene glycol 400 |
