5YHP

Proline iminopeptidase from Psychrophilic yeast glaciozyma antarctica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-03
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54178
Spacegroup name	P 21 2 21
Unit cell lengths	55.388, 82.269, 171.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.694 - 2.393
R-factor	0.1877
Rwork	0.186
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azw
RMSD bond length	0.008
RMSD bond angle	1.128
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.694
High resolution limit [Å]	2.393
Number of reflections	28758
<I/σ(I)>	6.12
Completeness [%]	90.8
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.1	30% PEG 4000, 0.2M Ammonium acetate, 0.1M Sodium citrate