5YHO
Crystal structure of acetolactate decarboxylase from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-27 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.832, 59.981, 80.818 |
| Unit cell angles | 90.00, 108.17, 90.00 |
Refinement procedure
| Resolution | 47.270 - 2.401 |
| R-factor | 0.2134 |
| Rwork | 0.210 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.270 |
| High resolution limit [Å] | 2.401 |
| Number of reflections | 16057 |
| <I/σ(I)> | 23.46 |
| Completeness [%] | 97.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Sodium acetate trihydrate pH4.5 3.0M Sodium chloride |
