5YHM
Crystal structure of dehydroquinate dehydratase with tris induced oligomerisation at 1.907 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.904, 155.606, 155.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.700 - 1.910 |
R-factor | 0.20044 |
Rwork | 0.200 |
R-free | 0.24779 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b6p |
RMSD bond length | 0.015 |
RMSD bond angle | 1.687 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.700 | 1.956 |
High resolution limit [Å] | 1.910 | 1.910 |
Rpim | 0.134 | |
Number of reflections | 139447 | 1715 |
<I/σ(I)> | 5.96 | |
Completeness [%] | 89.8 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5M Ammonium sulphate, 0.1M tris, 12% Glycerol |