5YHI

Crystal structure of YiiM from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	56.162, 84.837, 107.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.850
R-factor	0.20345
Rwork	0.201
R-free	0.24227
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o65
RMSD bond length	0.012
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.122	0.430
Number of reflections	12483	590
<I/σ(I)>	14.7	2.3
Completeness [%]	99.7
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.1M ammonium dihydrogen phosphate, 0.08M CHES, pH 9.5, 20% glycerol