5YHH

Crystal structure of YiiM from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-01
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	46.420, 51.526, 94.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.18714
Rwork	0.185
R-free	0.22346
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o65
RMSD bond length	0.015
RMSD bond angle	1.440
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.500
Number of reflections	15858
<I/σ(I)>	38.9	4.8
Completeness [%]	99.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.095M sodium citrate pH 5.6, 21% PEG 4000, 19% isopropanol, 5% glycerol