5YH5
The crystal structure of Staphylococcus aureus CntA in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2017-01-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 61 |
Unit cell lengths | 149.395, 149.395, 53.635 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.500 - 2.900 |
R-factor | 0.19369 |
Rwork | 0.190 |
R-free | 0.26367 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.046 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 15367 |
<I/σ(I)> | 16.7 |
Completeness [%] | 100.0 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M MES monohydrate pH 5.5, 25% (v/v) Polyethylene glycol 400 |